by Elsevier Science

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Cellulase refers to a class of enzymes produced chiefly by fungi, bacteria, and protozoans that catalyze cellulolysis. This volume of Methods in Enzymology comprehensively covers this topic. With an international board of authors, this volume covers subjects such as "The DNSA reducing assay for measuring cellulases," "Measuring processivity" and "In situ cellulose detection with carbohydrate-binding modules."

  • Comprehensively covers the topic of cellulases
  • International board of authors

Product Details

ISBN-13: 9780124159662
Publisher: Elsevier Science
Publication date: 07/10/2012
Series: ISSN , #510
Sold by: Barnes & Noble
Format: NOOK Book
Pages: 552
File size: 10 MB

Table of Contents

    1. Measurement of endo-1,4-b-glucanase
    2. Biomass Conversion determined via Fluorescent Cellulose Decay assay
    3. The analysis of saccharification in biomass using an automated high-throughput method
    4. Studies of enzymatic cleavage of cellulose using Polysaccharide Analysis by Carbohydrate gel Electrophoresis (PACE)
    5. Measuring processivity
    6. Distinguishing xyloglucanase activity in endo-b (1® 4) glucanases
    7. Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes
    8. The crystallization and structural analysis of cellulases (and other glycoside hydrolases): strategies and tactics
    9. Visualization of Cellobiohydrolase I from Trichoderma reesei Moving on Crystalline Cellulose Using High-Speed Atomic Force Microscopy
    10. Small angle X-ray scattering and crystallography; a winning combination for exploring the multi-modular organisation of cellulolytic macromolecular complexes
    11. Quantitative approaches to the analysis of carbohydrate-binding module function
    12. In Situ Detection Of Cellulose With Carbohydrate-Binding Modules
    13. Interactions between family 3 carbohydrate binding modules (CBMs) and cellulosomal linker peptides
    14. Approaches for improving thermostability characteristics in cellulases
    15. Thermophilic glycosynthases for oligosaccharides synthesis
    16. Engineering cellulase activity into Clostridium acetobutylicum
    17. Transformation of Clostridium thermocellum by electroporation
    18. Genetic and Functional Genomic Approaches for the Study of Plant Cell Wall Degradation in Cellvibrio japonicus
    19. Methods for the isolation of cellulose-degrading microorganisms
    20. Metagenomic approaches to the discovery of cellulases
    21. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes
    22. Measurements of relative binding of cohesin and dockerin mutants using an advanced ELISA technique for high-affinity interactions
    23. Designer cellulosomes for enhanced hydrolysis of cellulosic substrates
    24. High-throughput screening of cohesin mutant libraries on cellulose microarrays

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