ISBN-10:
0128211512
ISBN-13:
9780128211519
Pub. Date:
06/14/2020
Publisher:
Elsevier Science
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation

by Elsevier ScienceElsevier Science
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Overview

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.




  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on retinoid signaling pathways

Product Details

ISBN-13: 9780128211519
Publisher: Elsevier Science
Publication date: 06/14/2020
Series: Methods in Enzymology Series , #639
Pages: 430
Product dimensions: 6.00(w) x 9.00(h) x 0.94(d)

About the Author

David Chenoweth is at University of Pennsylvania, PA, USA

Table of Contents

    1. Fluorogenic detection of protein aggregates in live cells using the AggTag method
    2. Kwan Ho Jung and Xin Zhang

    3. Synthesis and application of a ratiometric probe for hydrogen peroxide
    4. Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains

    5. Protein labeling for FRET with methoxycoumarin and acridonylalanine
    6. Chloe M. Jones, Yarra Venkatesh and E. James Petersson

    7. Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays
    8. Paul T. Ludford III and Yitzhak Tor

    9. Method to discriminate amyloids using fluorescent probes
    10. Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang

    11. Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives
    12. Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas

    13. Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer
    14. John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo

    15. Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles
    16. Nanxi Wang and Lei Wang

    17. 4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy
    18. Arusha Acharyya, Ismail A. Ahmed and Feng Gai

    19. Dissecting contributions of catalytic and reader domains in regulation of histone demethylation
    20. Nektaria Petronikolou, James E. Longbotham and Danica Galonić Fujimori

    21. Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization
    22. Qing Zhang, Yue Zhang and Xuechen Li

    23. Chemical synthesis of di-ubiquitin modified histones for further biochemical studies
    24. Jia-Bin Li, Jun Liang and Changlin Tian

    25. Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies
    26. Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis

    27. FlICk (fluorescent isoindole crosslinking) for peptide stapling
    28. Mihajlo Todorovic and David M. Perrin

    29. Selective protein N-terminal labeling with N-hydroxysuccinimide esters
    30. Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey

    31. Covalent live-cell labeling of proteins using a photoreactive fluorogen
    32. Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra

    33. Photoconvertible diazaxanthilidene dyes for live cell imaging
    34. Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth

    35. No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag

Wei Sheng, James H. Geiger and Babak Borhan

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