Prairie Pioneers was one of a trio of "Three Mesquiteers" westerns directed by the forgotten Les Orleback. On this occasion, the Mesquiteers are enacted by Robert Livingston (as Stony Brooke), Bob Steele (Tucson Smith) and Rufe Davis (Lullaby Joslin). Their mission this time is to save young Roberto Ortega (Robert Kellard) from hanging for a crime he didn't commit. This requires our heroes to take on a gang of land-usurpers, headed by Carlos Montoya (Davison Clark). Along the way, Stony Brooke dallies romantically with Roberto's lovely sister Dolores (Esther Estrella).