| Basil Rathbone | Sherlock Holmes |
| Nigel Bruce | Dr. John H. Watson |
| Alan Mowbray | Maj. Duncan-Bleek / Col. Sebastian Moran |
| Kaaren Verne | Charlotte Eberli |
| Dennis Hoey | Inspector Lestrade |
| Lionel Atwill | Prof. Moriarty |
| Renee Godfrey | Vivian Vedder |
| William Post | Dr. Franz Tobel |
| Mary Forbes | Lady Margaret Carstairs |
| Billy Bevan | Train Attendant |
| Holmes Herbert | Sir Reginald Dailey |
| Frederic Worlock | Prof. William Kilbane |
| Mary Gordon | Mrs. Hudson |
| Leyland Hodgson | Conductor |
| Rudolph Anders | Braun |
Technical Credits
| Roy William Neill | Director |