| Kate Beckinsale | Selene |
| Stephen Rea | Dr. Jacob Lane |
| Michael Ealy | Detective Sebastian |
| Theo James | David |
| India Eisley | Eve |
| Sandrine Holt | Lida |
| Charles Dance | Thomas |
| Kris Holdenried | Quint |
| Jacob Blair | Officer Kolb |
| Adam Greydon Reid | Med Tech #1 |
| Catlin Adams | Olivia |
| Robert Lawrenson | Waterfront Cop |
| Lee Majdoub | Desk Guard #1 |
| John Innes | Medical Supervisor |
| Tyler McClendon | Scientist |
| Panou | Old City Cop #1 |
| Ian Rozylo | Old City Cop #2 |
| Benita Ha | Surgical Nurse |
| Christian Tessier | Security Guard #1 |
| Kurt Max Runte | Troop Leader |
| Mark Gibbon | Announcing Guard |
| Richard Cetrone | Lycan Creature #1 |
| Dan Payne | Lycan Creature #2 |
Technical Credits
| Mans Marlind | Director |
| Bjorn Stein | Director |
| Stuart Aikins | Casting |
| Deborah Aquila | Casting |
| Michael Babcock | Sound/Sound Designer |
| Paul Barry | Asst. Director |
| Allison Burnett | Screenwriter |
| David Clarke | Set Decoration/Design |
| David Coatsworth | Executive Producer |
| Sean Cossey | Casting |
| Eric Craig | Musical Direction/Supervision |
| Scott Dougherty | Executive Producer |
| Chris Duesterdiek | Sound Mixer |
| David Farmer | Sound/Sound Designer |
| Brendan Ferguson | Production Manager |
| Gary Myers | Art Director |
| Paul Haslinger | Score Composer |
| Douglas Higgins | Set Decoration/Design |
| John Hlavin | Original Story,Screenwriter |
| Martina Javorova | Art Director |
| David Kern | Executive Producer |
| Scott Kevan | Cinematographer |
| Gary Lucchesi | Producer |
| Todd Masters | Makeup Special Effects |
| Jeff McEvoy | Editor |
| Brian McNelis | Musical Direction/Supervision |
| James McQuaide | Executive Producer |
| Jay Mitchell | Set Decoration/Design |
| Claude Pare | Production Designer |
| Megan Poss | Set Decoration/Design |
| Monique Prudhomme | Costumes/Costume Designer |
| Eric Reid | Executive Producer |
| Tom Rosenberg | Producer |
| J. Michael Straczynski | Screenwriter |
| Skip Williamson | Executive Producer |
| Henry Winterstern | Executive Producer |
| Len Wiseman | Original Story,Producer,Screenwriter |
| Tricia Wood | Casting |
| Richard S. Wright | Producer |
