ISBN-10:
0792379829
ISBN-13:
9780792379829
Pub. Date:
10/31/2000
Publisher:
Springer US
Silencing, Heterochromatin and DNA Double Strand Break Repair / Edition 1

Silencing, Heterochromatin and DNA Double Strand Break Repair / Edition 1

by Kevin D. MillsKevin D. Mills

Hardcover

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Overview

The field of DNA repair is vast and advancing rapidly. Recent investigations have begun to focus on the involvement of chromatin in the repair of broken DNA. Although I have no doubt that many breakthroughs in our understanding of chromatin, chromatin regulation, and DNA repair lie in our future, presently this is a new line in inquiry. As such there are many, many unanswered questions. Indeed, most of the correct questions have probably not even been asked yet. Here I have attempted to present a review of some of the current body of knowledge that may prove relevant to understanding the role of chromatin in DNA repair. Because the volume of research, and the relevant findings, come from a staggering array of labs, systems, and ideas I have focused primarily on findings developed from the study of the budding yeast Saccharomyces cerevisiae. Unfortunately, this means that I have left out a great deal of information. It is my hope, however, that the information I do detail, particularly in Chapter 1, will give a flavor for the scope of the problem and perhaps highlight some of the interesting directions this field is taking, or may one day take. I would also point out that the primary research that is presented herein is not in any way meant to represent the comprehensive scope of research being performed. To understand DNA repair will require investigation from innumerable labs, performed by innumerable researchers, moving in unexpected directions.

Product Details

ISBN-13: 9780792379829
Publisher: Springer US
Publication date: 10/31/2000
Edition description: 2001
Pages: 122
Product dimensions: 6.10(w) x 9.25(h) x 0.01(d)

Table of Contents

Dedicationv
Table of Contentsvii
Forewordix
Acknowledgementsxi
Chapter 1Introduction to silencing, heterochromatin, and DNA break repair1
Introduction1
Yeast Silencing1
Silent HM loci2
SIR complex3
RAP14
ORC4
Histones5
Telomere silencing6
Telomere structure6
DNA Damage Repair8
Homologous recombination9
Single strand annealing11
Post replication repair12
The RAD6 epistasis group12
The RAD5 recombination regulator12
Nonhomologous end-joining13
Cell cycle checkpoint control15
Recent Findings19
Chapter 2Identification and characterization of high-copy antagonists of silencing in Saccharomyces cerevisiae21
Introduction21
Results23
Discussion31
Chapter 3MEC1-dependent redistribution of the Sir3 silencing protein from telomeres to DNA double strand breaks21
Summary37
Introduction38
Results39
Discussion49
Chapter 4The antisilencing gene ASF1 is required for resistance to DNA damage55
Introduction55
Results57
Discussion64
Chapter 5Summary and Conclusions71
Summary71
DNA repair72
Role of SIR complex73
Role of ASF174
Model for heterochromatin response75
Implications and questions75
Appendix AProcedures77
Appendix BYeast strains83
Appendix COligonucleotides85
References87
Index115

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